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Description:
SynaBlast™ (1.0.3) is a nucleotide/protein sequence searching and alignment application that searches the SynaBASE™ database platform and presents results in a BLAST-like output. By leveraging the exhaustive pattern structure of SynaBASE, very significant improvements in sensitivity and performance are observed whilst presenting the results in a familiar interface for online analysis.
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Application Example:
The protein encoded by the TGIF (TGFB-induced factor) is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains, which are highly conserved transcription regulators. Mutations in this gene are associated with holoprosencephaly type 4, a structural defect of the developing forebrain in humans. In this example, we will use the TGIF transcript variant 8 (an alternatively spliced version of which there are 8 in total) to map its position to the human genome (NCBI36) and deduce its exon positions. Using a 1.5Kbp query, SynaBlast was able to search a 3 billion bp SynaBASE in less than ¼ of a second. |
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To run the example above please:
- Go to
www.mgrc.com.my.
- Click on “SynaBlast”.
- Click on “Test Sequence” and select the Newsbyte test sequence labelled “Homo sapiens TGFB-induced factor transcript variant 8” by clicking on the “Copy” button next to the sequence.
- The “Homo sapiens genome (NCBI build36 v1)” SynaBASE should be selected.
- Using the default parameters given, click on “SynaBlast” to begin your analysis.
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Figure 1: The query submission page of SynaBlast (1.0.3). |
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Figure 2: SynaBlast results page showing the top ranking result mapping to chromosome 18. |
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Test Sequence
>gi|28178856|ref|NM_174886.1| Homo sapiens TGFB-induced factor (TALE family homeobox) (TGIF),
transcript variant 8, mRNA
CAAAGGCCAGGCCTGACGGAAGAGCCCAGCCTTCGCCGAGGGCCGCAGGGAGCCAGAGACTCGAGCCTGCGGTTCCAG
CCCCGCCGCGGTTCCGGCCCCGCCGCGATCCCGAGGCCTGCGCATGCTCGGTGCTGCGCGGGGCTCCCTCCTTACCGC
AGGAGCTCCTCATCGAGAAACCCAGGTTGGTGTCTTTTCCACGTGGTAGAAGCTGGCTAACCTCCACTTCCACATTCC
AGCCCAAGGGAAAGTCCAAGGTATTGTTGCAGCATCTGGCAGTGAGACTGAGGATGAGGACAGCATGGACATTCCCTT
GGACCTTTCTTCATCCGCTGGCTCAGGCAAGAGAAGGAGAAGGGGCAACCTACCCAAGGAGTCTGTGCAGATTCTTCG
GGATTGGCTGTATGAGCACCGTTACAATGCCTATCCTTCAGAGCAAGAAAAAGCGTTGCTGTCCCAGCAAACACACCT
GTCTACGCTACAGGTCTGTAACTGGTTCATCAACGCCCGCCGCAGGCTCCTCCCTGACATGCTGAGAAAGGATGGCAA
AGATCCAAATCAGTTCACAATTTCCCGCCGTGGGGCCAAGATTTCTGAAACGAGCTCTGTGGAGTCCGTGATGGGCAT
CAAAAACTTCATGCCAGCTCTAGAGGAGACCCCATTTCATTCCTGTACAGCTGGGCCAAACCCAACCCTAGGGAGGCC
ACTGTCTCCTAAGCCGTCATCCCCGGGATCAGTTTTGGCTCGTCCATCAGTGATCTGCCATACCACTGTGACTGCATT
GAAAGATGTCCCTTTCTCTCTCTGCCAGTCGGTCGGTGTGGGACAAAACACAGATATACAGCAGATAGCGGCCAAAAA
CTTCACAGACACCTCTCTCATGTACCCAGAGGACACTTGTAAATCTGGACCAAGTACGAATACACAGAGTGGTCTTTT
CAACACTCCTCCCCCTACTCCACCGGACCTCAACCAGGACTTCAGTGGATTTCAGCTTCTAGTGGATGTTGCACTCAA
ACGGGCTGCAGAGATGGAGCTTCAGGCAAAACTTACAGCTTAACCCATTTTCAAGCAAAACAGTTCTCAGAAATGTCA
TGATTGCCGGGGTGAAGGCAAGAGATGAATTGCATTATTTTATATATTTTTTATTAATATTTGCACATGGGATTGCTA
AAACAGCTTCCTGTTACTGAGATGTCTTCAATGGAATACAGTCATTCCAAGAACTATAAACTTAAAGCTACTGTAGAA
ACAAAGGGTTTTCTTTTTTAAATGTTTCTTGGTAGATTATTCATAATGTGAGATGGTTCCCAATATCATGTGATTTTT
TTTTTCCTCCCCTTCCCTTTTTTTGTTATTTTTTCAGACTGTGCAATACTTAGAGAACCTATAGCATCTTCTCATTCC
CATGTGGAACAGGATGCCCACATACTGTCTAATTAATAAATTTTCCATTTTTTTTCAAACAAGTATGAATCTAGTTGG
TTGATGCCTTTTTTTTCATGACATAATAAAGTATTTTCTTTAAAAAAAAAAAAAAAAA
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